Hydroxyalkyl esters of quinoxaline-di-n-oxide-2-carboxylic acid

ABSTRACT

Novel alkyl esters of quinoxaline-di-N-oxide-2-carboxylic acid substituted on the alkyl portion of the ester by hydroxy, acyloxy, n-alkyl carbamyloxy, dialkylaminoacyloxy, carboxyacyloxy, alkoxycarbonyloxy, haloacyloxy, amino and monoand disubstituted amino, useful as antibacterial agents and in promoting growth and improving feed efficiency of animals in general.

United States Patent [19.1

Cronin et a1.

[ June 18, 1974 HYDROXYALKYL ESTERS OF QUINOXAImE-DI-N-OXIDEJ- CARBOXYLIC ACID [75] Inventors: Timothy H. Cronin, East Lyme;

Kenneth Richardson, Groton, be of Conn.

[73] Assignee: Pfizer Inc., New York, NY.

[22] Filed: Apr. 20, 1971 [21] Appl. No.: 135,792

Related US. Application Data [63] Continuation-impart of Ser. No. 20,841, March 18,

1970, abandoned.

[52] US. Cl. 260/250 R, '260/244 R, 260/251, 260/307 F, 260/482 C, 260/488 J, 260/583 G, 424/250 [51] Int. Cl C07d 51/78 [58] Field of Search 260/250 R, 250 ON [56] I References Cited UNITED STATES PATENTS 2,921,937 1/1960 Gordon et a1 260/267 3,344,022 9/1967 Johnston l67/53.1 3,753,987 8/1973 Cronin et a1. 260/250 R 3,759,912 9/1973 Derungs 260/250 R 3,763,162 10/1973 Kasubick 260/250 R FQREIGN PATENTS OR APPLICATIONS 3,717M 4/1964 France 260/250 R OTHER PUBLICATIONS Burger, Medicinal Chemistry, Wiley lnterscience, New York, 1960, pp. 64, 69, 70. R.S.403.B8.

Noller, Chemistry of Organic Compounds, Saunders and Co., Philadelphia, 1965, pp. 97, 472474. Landquist et al., J. Chem. Soc., 2052 (1956). Derungs, Chemical Abstract 75:98591q of Ger. Offen. No. 2,059,824.

Primary Examiner-Donald G. Daus Assistant Examiner-Ralph D. McCloud Attorney, Agent, or Firm-Connolly and Hutz [57] ABSTRACT Novel alkyl esters of quinoxaline-di-N-oxide-Z- carboxylic acid substituted on the alkyl portion of the ester by hydroxy, acyloxy, n-alkyl carbamyloxy, dialkylaminoacyloxy, carboxyacyloxy, alkoxycarbonyloxy, haloacyloxy, amino and monoand disubstituted amino, useful as antibacterial agents and in promoting growth and improving feed efficiency of animals in general.

8 Claims, No Drawings HYDROXYALKYL ESTERS OF QUINOXALINE-DI-N-OXIDEZ-CARBOXYLIC ACID Cross-Reference to Related Applications This application is a continuation-in-part of copending application Ser. No. 20,841, filed Mar. 18, 1970, and now abandoned.

cfiafiiiing efforts to uncover new and more useful antibacterial agents have lead, over the years, to the dev elopment of a wide variety of prototype organic compounds including numerous congeners of quinoxalinedi-N-oxides. Landquist, et al., J. Chem. 500., 2052 (1956), in a search for compounds of improved antibacterial or antiprotozoal activity, reported the preparation of several derivatives of 2-methyland 2,3- dimethylquinoxaline-diN-oxides in which the methyl groups were converted to groups such as bromomethylacetoxymethyl' and hydroxymethyl including 3- methyl-2-carbethoxyquinoxaline-di-N-oxide. However, no utility is alleged for any of these compounds. French Pat. No. M3717, granted Jan. 3, 1966, discloses 2- quinoxalinecarboxamide-di-N-oxides in which the carboxamide group may be substituted with an alkyl, substituted alkyl, aryl, aralkyl, or cycloalkyl group. Also disclosed. but the structure not indicated, are the corresponding 2-quinoxalinecarboxylic acid substituted esters. They are reported to be of use in human therapy as antitubercular, antibacterial, anticancer, antivirus and antiprotozoal agents.

W Belgian Pat. No. 697,976 granted Nov. 3, 1967, de-

SUMMARY-OF This lNvTSNTION The novel antibacterial and growth 5655a quinoxaline-di-N-oxides of this invention are represented by the formula:

0 1, oo=-A-z X l 0 "wiiaraix'rs a substituent at the 6- or 7 position se lected from the group consisting of hydrogen, fluorine, chlorine, bromine, trifluoromethyl, methyl and methoxy;

A is alkylene of from -2 to 5 carbon atoms; and

Z is selected from the group consisting of: (l) bydroxy; (2) acyloxy of the formula --O CR wherein R is selected from the group consisting of hydrogen; alkyl containing from one to 10 carbon atoms; alkoxy containing from one to four carbon atoms; alkylamino containing from one to four carbon atoms; carboxyalkyl containing from two to eight carbon atoms; and substituted alkyl wherein said alkyl contains from one to three carbon atoms and said substituent is selected from the group consisting of chlorine, bromine and dialkylamino wherein said alkyl contains from one to three carbon atoms; (3) amino; (4) monosubstituted amino of the formula NHR, wherein R, is alkyl containing from one to four carbon atoms; (5) disubstituted amino of the formula NR R wherein R and R are each alkyl containing from one to two carbon atoms;

acid addition salts thereof wherein Z is selected from the group consisting of amino, monosubstituted amino, disubstituted amino, and dialkylaminoacyloxy; pharmaceutically acceptable basic salts thereof wherein Z is carboxyacyloxy; and lower alkyl pharmaceutically acceptable quaternary salts thereof wherein Z is dialkylaminoacyloxy.

Of particular interest, because of their in vitro and in vivo antibacterial activity and animal growth promotant activity, are compounds wherein X is hydrogen or chlorine, A is alkylene of from two to three carbon atoms and Z is hydroxy, acetoxy, n-octanoyloxy, (ethoxycarbonyhoxy, 3-carboxypropiony1oxy, 4- carboxybutyryloxy, amino, dimethylamino or methylethylamino.

Also considered within the scope of this invention are congeners of the aforementioned compounds where the 3-methyl substituent is replaced by lower alkyl containing from two or three carbon atoms; where Z is acyloxy of the formula -O CR, wherein R is phenyl and substituted phenyl, the substituent being selected from the group consisting of halo (F, Cl, Br), lower alkoxy, lower alkyl and lower dialkylamino; where A is part of a cycloalkyl group containing from three to eight carbon atoms and where A, as alkylene or part of a cycloalkyl group is substituted by hydroxy. Also included within the purview of the present application are those congeners wherein the acyl portion of the carboxyacyloxy group, Z, is derived from a cycloalkylcarboxylic acid or an aromatic acid, including benzoic acids, napthalenecarboxylic acids and heterocyclic acids. Also contemplated is polycarboxyacyloxy as being represented by the variable Z. In like manner these compounds also possess antibacterial and growth promotant activity.

DETAILEDDESCRIP'I'ION OF THE INVENTION In accordance with the process employed for preparing' 3-methyl-2-quinoxalinecarboxylic acid alkyl esters of the instant invention, an appropriately substituted benzofuroxan is contacted with an acetoacetic ester deriyat vs iillqstr si .fihsfv n s he a;

wherein X and A are as previously defined, and Z is alkanoyloxy or disubstituted amino.

As a necessary element of the herein described process, the reaction sequences described above must be effected in the presence of a base. Such a base is of varied character. For instance, it is meant to contemplate such bases as organic amines, ammonia, alkali metal hydroxides, hydrides and alkoxides. Representative of such bases are ammonia, primary amines such as npropylamine, n-butylamine, aniline, cyclohexylamine, benzylamine, p-toluidine, ethylamine, octylamine, tertiary amines such as diethylaniline, N- methylpyrrolidine, N,N-dimethylpyrimidine, N- methylmorpholine, and l,S-diazabicyclo-l4,3,01-- nonene; sodium hydroxide, potassium hydroxide, ammonium hydroxide, sodium ethoxide, potassium methoxide, and sodium hydride. The preferred base to use is an organic amine, ammonia or alkoxide.

In practice, a solution or suspension of the appropriately substituted benzofuroxan and the requisite acetoacetate in a reaction-inert solvent such as ethanol, N,N-dimethylformamide, benzene, tetrahydrofuran, chloroform or hexamethylphosphoramide is treated with an alkoxide, e.g., sodium ethoxide. It is preferable to use at least an equimolar amount of the benzofuroxan and acetoacetate, while the amount of base may be from a catalytic to equimolar amount. The reaction is carried out at ambient temperatures, although it may be heated to 100 C. to hasten product formation. Reaction time is not critical, but will vary depending on the reactivity of the starting materials, temperature and solvent employed. Substantial yields of the desired products are isolated with reaction periods of minutes to 24 hours.

The requisite benzofuroxans and acetoacetates are either readily available or easily prepared by those skilled in the art. For instance, the synthesis of variously substituted bcnzofuroxans is described by Kaufman, et al., in Advan. Heleroqvclic Chem, 10, l 1969). Acetoacetates are readily prepared from diketene according to the general procedure of Brintzinger, et al., Chem. Ber., 83, 103 (I950).

Quinoxaline-di-N-oxides of the instant invention result from the condensation of benzofuroxan and substituted benzofuroxans with acetoacetic esters such that the 2- and 3-positions of the resulting annellated structure represent the carbonyl-carbon and the carbon of the active methylene group of the acetoacetate.

The substituents on the benzene moiety of the 3- methyl-Z-quinoxalinecarboxylic acid estersl ,4 dioxides can vary widely. For example, at least one of the following substituents can be present: hydrogen, methyl, methoxy, chloro, fluoro, bromo and trifluoromethyl. In like manner, the substituents may include methylthio, methylsulfonyl, methylsulfinyl, trifluoromethylthio, trifluoromethoxy, acetyl, amino, nitro, dimethylamino, acetamido, sulfamyl and monoand dimethylsulfamyl, methylsulfonylamino, mercapto, hydroxy, acetoxy, carboxy, carboxamido and monoand dimethylcarbox amido, cyano, aldehydo and phosphono. The favored positions on the fused benzene ring of said final products are the 6- or 7-positions. The favored positions for substituents on the aryl ring of the starting benzofuroxans leading to said final products are the 5- or 6- positions. When one of said substituted benzofuroxans is condensed with the requisite acetoacetate, a 6- and 7-substituted quinoxaline-di-N-oxide are produced. This multiple product formation results because of the two orientation possibilities of the acetoacetate fragment in the final product. For example, if one reacts a 5 -substituted l nzofuroxan of the formula:

with a reactant CH;,COCH CO A-Z, two products, a 6- and 7-substituted quinoxaline'di-N-oxide, result as shown by the formulae:

If the corresponding 6-substituted benzofuroxan is employed as the starting material, the same two possible products are formed.

The mixture of isomers is recovered by methods known to those skilled in the art. In many of the preparations disclosed wherein a solid, often crystalline material, separates from the reaction mixture, the solid appears to consist predominantly of one of the isomers. Said isomer can be purified by repeated recrystallization from a suitable solvent to a constant melting point. The other isomer, the one present in smaller amounts in the originally isolated solid material, is the predominant product in the mother liquor. It can be recovered therefrom by methods known to those skilled in the art, as for example, the evaporation of the 5v zation or trituration thus allows the separation of the mixture of positional isomers from such extraneous materials as starting material and undesirable by-products.

The identification of the isomers has not been completed. Both isomers of a given compound, however, exhibit the same type of activity, e.g., as animal growth promotants or as antibacterial agents.

Compounds of the present invention wherein Z is hydroxy are most conveniently prepared from the requisite acyloxy analogs by acid hydrolysis. In practice, a 3-methyl-2-quinoxalinecarboxylic acid alkyl ester, 1,4- dioxide substituted in the alkyl portion of the ester by an alkanoyloxy moiety is added to an aqueous acid solution, for instance, sulfuric, phosphoric, or hydrochloric. In general, the concentration of the acid is from about l-l2N, with a preferred range of lO-lZN. The hydrolysis is effected at temperatures of from 50 C. with a preferred temperature of 2535 C. and a reaction period of 30 minutes to 3 hours. After the reaction is completed water is added to the mixture and the pH is adjusted to pH 5 using an aqueous solution of a suitable base, e.g., sodium hydroxide. The mixture is then extracted several times with a suitable solvent such as chloroform, and the organic layer separated, dried over sodium sulfate and concentrated in vacuo to dryness.

Compounds of the present invention wherein Z is amino, NH2, ,are synthesized by the following scheme:

Hydrolysis of the aforementioned oxazacyclic moiety at the 2-position of the appropriate 3 methylquinoxalinel ,4-di-N-oxide is most conveniently carried out using a suitable acid, such as hydrobromic, hydrochloric, phosphoric or sulfuric in a water-water miscible solvent system such as methanol-water or ethanol-water. In general, a two to five fold excess of said acid is employed to facilitate the reaction. The hydrolysis is carried out at temperatures of 0-50 C. with a preferred range of 25-35 C. and for a reaction pe- V riod of minutes to 2 hours.

The workup procedure for said reaction consists of removal of excess water, solvent and acid under reduced pressure, followed by trituration of the resulting salt with a suitable solvent, such as ethyl acetate or isopropanol. It is advantageous, whenever possible, to employ the same acid for the hydrolysis as is desired as the salt of the final product. For example, if the hydrochloride salt is desired then hydrochloric acid is employed, the sulfate saltsulfuric acid, etc.

The requisite 2-( l,3-oxazacyclic)-3- methylquinoxaline-l ,4-di-N-oxides for the aforementioned hydrolysis reaction are conveniently prepared by one of two synthetic routes. The first scheme, as illustrated, consists of the C A sl/ J material; or as a propriately sursrnmewaa zofuroxan with an acetoacetamide substituted on the amide nitrogen by an w-haloalkylene moiety (Z C1 or Br). The previously outlined reaction conditions leading to the products of the instant invention are employed with the exception that at least two molar equivalents of base are used per mole of benzofuroxan and acetoacetamide. In general, the workup procedure consists of cooling the reaction mixture and filtering the resulting product or, alternately, pouring the mixture into water followed by extraction of the product with an appropriate solvent, such as chloroform or methylene chloride.

The N-substituted acetoacetamide derivatives employed as starting materials are easily synthesized by those skilled in the art as taught by the method of DAngeli, et al., Tetrahedron Letters, 605 1965).

The second and alternate synthesis of the intermediate 2-( l,3-oxazacyclic)-3-methylquinoxalinel ,4-di-N- oxides comprises contacting an appropriately substituted benzofuroxan with a 2-acetonyl-l,3-oxazacyclic compound, as illustrated:

ThEieaction conditions, solvents afia'sai'siiairiading to these compounds are the same as previously outlined for the products of the present invention.

Acylation of the anion of the appropriate 2-methyl- 1,3-oxazacyclic intermediate employing either ethyl acetate or acetyl chloride provides the desired 2- acetonyl derivative. In practice, the anion of the requisite 2-methyl-l,3-oxazacyclic compound generated according to the conditions as taught by Meyer, et al., J. Am. Chem. Soc., 91, 763 (1969), is treated with an equimolar amount of ethyl acetate or acetyl chloride in a suitable solvent such as tetrahydrofuran or diethyl ether at temperatures of from 30 to -50 C. When the reaction is complete, it is filtered and the solvent removed invacuo. The desired acetonyl derivative is purified either by distillation under, reduced pressure or recrystallized from a suitable solvent.-

The aforementioned 2-methyl-l,3-oxazaeyclic compounds necessary for the aforedescribed procedure are either readily available or easily prepared by methods known to those skilled in the art, for instance, according to the methods as outlined by Elderfield, Heterocyclic Compounds," John Wiley and Sons, Inc., New

York, l957, Vol. 5, page 377 and Vol. 6, page 534, and g by Eckstein, Adv. Heterocyclic Chem., 311 (1963).

Compounds of the instant invention where Z is a sec on dary rnine lfll l ll wherein R is as previously in- Reaction conditions, solvents and isolation procedures of the first reaction relating to the formation of the quinoxaline-di-N-oxide from the requisite benzofuroxan and acetoacetate are as previously described, as are the reaction conditions leading to the acetoacetates from diketene and the appropriate N-tbutyloxycarbonylaminoalkanol.

The aforementioned N-tbutyloxycarbonylaminoalkanols are easily prepared from the aminoalkanols and t-butyl cyanoformate by procedures known in the art; for example, by the general procedure of Leplawy, et al., Bull. acad. polon. sci., ser. sci. chem., l2, 2l (I964); C. A., 61, 1933 (1964).

The second step in the sequence which relates to the removal of the t-butyl protecting group is carried out using a dilute mineral acid such as hydrochloric acid or hydrobromic acid. In practice, the 3-methyl-2-quinoxalinecarboxylic acid, N-t-butyloxycarboxylaminoalkylene ester, 1.4-dioxide in a suitable, water miscible solvent such as ethanol or methanol is treated with at least one mole equivalent of said acid and preferably a l-50 percent excess. The reaction is carried out in a temperature range of 0-5 0 C., with a preferred reaction temperature of 2535 C., and a reaction period of 30 minutes to 3 hours. A convenient method of isolation consists of removal of the solvent and excess acid under reduced pressure. The resulting salt can be further purified by recrystallization from or trituration with a suitable solvent.

In addition to the aforementioned synthetic routes to the compounds of the present invention wherein Z is acyloxy and disubstituted amino, there are three additional preparative schemes.

Scheme l encompasses the condensation of a benzofuroxan with an a keto ester, illustrated as follows N O X O B4CHalLCOz-A-Z 8 wherein X andxare as previously indicated, R is alkyl containing from one to three carbon atoms and Z is acyloxy of the formula O CR where R is hydrogen, alkyl containing from one to 10 carbon atoms, or benzoyl and substituted benzoly; Z is disubstituted amino.

The reaction conditions and solvents for Scheme 1 are essentially those described previously for the condensation of a benzofuroxan and an a'cetoacetate ester, with the preferred base an alkali metal alkoxide. The work-up procedure and product isolation are as previously indicated.

The a-ketoesters employed as the starting reagents for the aforedescribed reaction are conveniently prepared by one skilled in the art, e.g., according to' the method as outlined by Vogel, et al., Helv. Chim. Acta., 33, 1231 (1950).

The second alternate route, Scheme ll, comprises a transesterification reaction of a preformed 3-alkyl(C C )-2-quinoxalinecarboxylic acid ester with an alkanol of the formula HO--A--Z wherein A and Z are as .iad ateslia we asi i s r and M is lower 'a l020 percent excess. The reaction may be carried out either in a solvent such as benzene, toluene or xylene or neat, i.e., without a solvent. However, it is preferred that a solvent be used to provide a single contact phase. The reaction temperature used will vary with the reflux temperature of the solvent employed. In general, temperatures of l 10 C. are adequate to effect the aforementioned reaction, with reaction times dependent on the dilution of the reaction mixture and the reactivities of the starting reagents. The products are isolated by removing the solvent in vacuo, followed by trituration or recrystallization from an appropriate solvent. The requisite 3-alkyl-2-quinoxalinecarboxylic acid esters used as a starting reagent in Scheme ll are prepared according to the method of lssidorides, et al., J. Org. Chem, 31, 4067 (1966).

The third alternate preparative method, Scheme lll, employs the alcoholysis of the corresponding 2- cyano quinoxaline-di-N-oxides asfollows:

wherein X, R A and Z areas previously indicated.

in practice, the nitrile is heated with atiatah equimolar amount and as much as a 100 percent excess of t requisite k nolin the W Q t least asse t: molar amount plus as much as a 20 percent excess of an acid such as sulfuric or hydrochloric acid in a reaction-inert solvent or neat. A necessary element in the described reaction is at least an equimolar amount of water. In general, steam bath temperatures are adequate to effect said reaction. Reaction times are not critical, but will vary with temperature and the reactivof thestarting reagents.

John Wiley& Sons, lnc., New York,- 1956, page 348 Compounds of the instant invention wherein Z is carboxyacyloxy and X and A are as previously indicated are synthesized from those wherein Z is hydroxy via acylation of said hydroxy moiety employing an activated form of a dicarboxylic acid. Said activation may be through the acid halide, mixed anhydride or the simple cyclic anhydride, all in the presence of a proton acceptor such as a tertiary amine, i.e., triethyl amine. Also useful in the aforedescribed condensation utilizing the dicarboxylic acid and alcohol is the condensing agent QiEQCEUL dQ. W. .7

In practice, the hydroxyalkyl ester of 3-methyl-2- quinoxalinecarboxylic acid l,4-dioxide is acylated with at least an equimolar amount, plus as much as a 100 percent excess of an activated dicarboxylic acid selected from the group previously enumerated. Said reaction is conducted in an aprotic solvent such as acetone, methylene chloride or chloroform. Reflux temperatures are favored, with reaction times of 2-6 hours. As previously mentioned, a proton acceptor is employed in molar amounts equivalent to the dicarboxylic a id: .s The reaction is subsequently quenched in water, extracted with a water immiscible solvent and the water layer acidified with concentrated hydrochloric acid to a pH of about 3. The liberated product is extracted into chloroform and the organic layer separated, dried over sodium sulfate and concentrated in vacuo to dryness. The residual product can be further purified by recrystaflization from an appropriate solvent.

Acylation of the hydroxy] moiety with alkyl chloroformate esters leads to those analogs wherein Z is alkoxycarbonyloxy, and is carried out employing the appropriate hydroxyalkyl ester and a halo formate ester in a solvent such as chloroform or methylene chloride in the presence of a tertiary amine, e.g., pyridine or triethyl amine. Generally, it is advantageous to employ as much as a 100 percent excess of the halo formate and tetuqu amine-W V. a

Experimentally, a solution of the hydroxyalkyl ester and tertiary amine, cooled to C. is treated with the requisite halo formate. After 1-2 hours at ambient temperatures the reaction mixture is treated with a saturated sodium bicarbonate solution then washed with 6 N hydrochloric acid. The solvent, containing the product, is then dried and evaporated to dryness.

sui ah i t leut r In a similar manner, congeners of the products of the present invention wherein Z is chloroor bromoacyloxy and Nalkylcarbarnyloxy are synthesized by contacting the requisite hydroxyalkyl ester with a chloroor bromoacyl halide or alkylisocyanate, respectively.

Reaction of the aforementioned chloroor bromoacyloxy compounds with at least two moles of a secondary amine in a reaction-inert solvent such as benzene, chloroform or methylene chloride at 5080 C. for 1-3 hours results in the preparation of those congeners wherein Z is dialkylaminoacyloxy.

These resulting tertiary amines are readily quaternarized by treatmentwith at least an equimolar amount of an appropriate alkylating agent, e.g., alkyl halide or alkyl sulfonate ester. Said reaction is most conveniently carried out at elevated temperatures in a reaction-inert solvent such as acetone, tetrahydrofuran or benzene. The product, which generally forms as a precipitate as the reaction proceeds, is filtered from the cooled reaction mixture, and subsequently recrystallized.

An alternate, and equally effective means of preparing these quaternary salts, comprises the substitution of a tertiary amine for a secondary amine in the aforementioned reaction with compounds wherein Z is chloroand bromoacyloxy. In said reaction, compounds of the present invention wherein Z is chloroand bromoacyloxy dissolved in methylene chloride or chloroform are treated with at least a molar equivalent, plus as much as a percent excess, of the appropriate trialkylamine at ice-bath temperatures for from 6-12 hours. The resulting quaternary chloride or bromide salts are conveniently isolated by filtration from the reaction mixture.

As has been previously noted, the compounds of the instant invention wherein Z is amino, monoand disubstituted amino and dialkylaminoacyloxy can form acid addition salts wherein said acid addition salts are considered to be the full equivalent of the free bases. Basic compounds of the present invention are converted to the acid addition salts by interaction of the base with an acid either in an aqueous or non-aqueous medium. In a similar manner, treatment of the acid addition salts with an aqueous base solution, e.g., alkali metal hydroxides, alkali metal carbonates and alkali metal bicarbonates or with a metal cation which forms an insoluble precipitate with the acid anion, results in a regeneration of the free base form. Such conversions are best carried out as rapidly as possible and under temperature conditions and method dictated by the stability of said basic products. The bases thus regenerated may be reconverted to the same or a different acid addition salt.

In the utilization of the chemotherapeutic activity of those compounds of the present invention which form salts, it is preferred, of course, to use pharmaceutically acceptable salts. Although water-insolubility, high toxicity, or lack of crystalline nature may make some particular salt species unsuitable or less desirable for use as such in a given pharmaceutical application, the

water insoluble or toxic salts can be converted to the corresponding pharmaceutically acceptable bases by decomposition of the salt as described above, or alternately they can be converted to any desired pharmaceutically acceptable acid-addition salt.

Examples of acids which provide pharmaceutically acceptable anions are hydrochloric, hydrobromic, hydroiodic, nitric, sulfuric, or sulfurous, phosphoric,

1 l acetic, lactic, citric, tartaric, succinic, maleic and gluconic.

A characteristic feature of the compounds of the present invention wherein Z is carboxyacyloxy and A and X are as previously indicated is their ability to form basic salts. Said compounds are converted to basic salts by the interaction of said acid with an appropriate base in an aqueous or nonaqueous medium. Such basic reagents suitably employed in the preparation of said salts can vary in nature, and are meant to contemplate such bases as organic amines, ammonia, alkali metal hydroxides, carbonates, bicarbonates, hydrides and alkoxides,

as well as alkali earth metal hydroxides, hydrides, al-

koxides and carbonates. Representative of such bases are ammonia, primary amines such as n-propylamine, n-butylamine, aniline, cyclohexylamine, benzylamine, p-toluidine, ethylamine, octylamine, tertiary amines such as diethylaniline, N-methylpyrrolidine, N- methylmorpholine and l,5-diazabicyclo-[4,3,0]-- nonene; sodium hydroxide, potassium hydroxide, ammonium hydroxide, sodium ethoxide, potassium methoxide, magnesium hydroxide, calcium hydride and barium hydroxide.

As previously indicated, the quinoxaline-di-N-oxides of the present invention are all readily adapted to therapeutic use as antibacterial agents and as growth promotants. Typical member compounds of interest in this series include 3-methyl-2-quinoxalinecarboxylic acid, 2-aminoethyl ester, l,4-dioxide; 3-methyl-2- quinoxalinecarboxylic acid, 2-(dimethylamino)ethyl ester, l,4-dioxide; 3-methyl-2-quinoxalinecarboxylic acid, Z-(methylethylamino)ethyl ester, l,4-dioxide, 3- methyl-Z-quinoxalinecarboxylic acid, 2- (acetyloxy)ethyl ester, l,4-di0xide; 3-methyl-2- quinoxalinecarboxylic acid, 2-hydroxyethyl ester, l,4- dioxide; 3-methyl-2-quinoxalinecarboxylic acid, 2-(3- carboxypropionyloxy)ethyl ester, l,4-dioxide; 3- methyl-2-quinoxalinecarboxylic acid, 2-(4-carboxybutyryloxy)ethyl ester, l,4-dioxide; 3-methyl-2- quinoxalinecarboxylic acid, 2-(octanoyloxy)ethyl ester, l,4-dioxide; 3-methyl-2-quinoxalinecarboxylic acid. 2-[(ethoxycarbonyl)oxylethyl ester. l,4-dioxide; 3-methyl-2-quinoxalinecarboxylic acid, 3- (acetyloxy)propyl ester, l,4-dioxide, 3-methyl-2- quinoxalinecarboxylic acid, 3-hydroxypropyl ester, l,4-dioxide; 3-methyl-2-quinoxalinecarboxylic acid,

- l-methyl-2-hydroxyethyl ester, l,4-dioxide; 3-methyl- 2-quinoxalinecarboxylic acid, 3- (dimethylamino)propyl ester, l,4-dioxide; 3-methyl-6- and 7-chloro-2-quinoxalinecarboxylic acid, l-methyl- 2-(diethylamino)ethyl ester, l,4-dioxide, and S-methyl- 6- and 7-chloro-2-quinoxalinecarboxylic acid, 2- (acetyloxy)ethyl ester, l,4-dioxide.

The valuable products of this invention are remarkably effective in treating a wide variety of pathogenic micro-organisms. They are, therefore, useful as industrial antimicrobials, for example, in water treatment, slime-control, paint preservation and wood preservation as well as for topical application purposes as disinfectants.

For in vitro use, e.g., for topical application, it will often be convenient to compound the selected product with a pharmaceutically acceptable carrier such as vegetable or mineral oil or an emollient cream. Similarly, they may be dissolved or dispersed in liquid carriers or solvents such as water, alcohoLglycols or mixtures thereof or other pharmaceutically acceptable inert media, that is, media which have no harmful effect on the active ingredient. For such purposes, it will generally be acceptable to employ concentrations of active ingredients of from about 0.01 percent to about 10 percent by weight based on total composition.

In determining the in vitro activity of an antibiotic, the sensitivity of the various micro-organisms to an an tibiotic is determined by the commonly accepted twofold serial dilution technique. Final concentrations of compound per ml. range from 100 mcg. in the first tube to 0.19 mcg. in the tenth tube, The inoculum consists of 0.5 ml. of a l X 10 dilution of a standardized culture. Final volume in each tube or cup in the DisPoso tray is 1.0 ml. The tubes are incubated at 37 C. for approximately 24 hours. The medium used is Witkins synthetic or Brain Heart Infusion (BHl). The sensitivity (MIC minimal inhibitory concentration) of the test organism is accepted as evidenced by the absence of gross turbidity.

Further, compounds described herein exhibit useful broad spectrum activity, that is, activity against both gram-negative and gram-positive bacteria, in contrast to the usual gram-negative activity of quinoxaline-di-N- oxides. Additionally, the compounds of the present invention are active in vivo and are especially useful as animal growth promotants, especially for swine and poultry.

When used in vivo for such purposes, these novel compounds can be administered orally or parenterally, e.g., by subcutaneous, intramuscular or intravenous injection, at a dosage of from about l mg./kg. to about 100 mgJkg. of body weight. Vehicles suitable for parenteral injection may be either aqueous such as water, isotonic saline, isotonic dextrose, Ringers solution, or non-aqueous such as fatty oils of vegetable origin (cotton seed, peanut oil, corn, sesame), dimethylsulfoxide and other non-aqueous vehicles which will not interfere with therapeutic efficiency of the preparation and are nontoxic in the volume or proportion used (glycerol, propylene glycol, sorbitol and dimethylacetamide). Additionally, compositions suitable for extemporaneous preparation of solutions prior to administration may advantageously be made. Such compositions may include liquid diluents, for example, propylene glycol, diethyl carbonate, glycerol, sorbitol, etc.; buffering agents, hyaluronidase, local anesthetics and inorganic salts to afford desirable pharmacological properties. These compounds may also be combined with various phannaceutically acceptable inert carriers including solid diluents, aqueous vehicles, nontoxic organic solvents in the form of capsules, tablets, lozenges, troches, dry mixes, suspensions, solutions, elixirs and parenteral solutions or suspensions. In general, the compounds are used in various dosage forms at concentration levels ranging from about 0.5 percent to about percent by weight of the total composition.

The in vivo efficacy of the compounds of the instant invention is determined by the antibacterial activity against acute infections in mice. The acute experimental infections are produced by the intraperitoneal inoculation of standardized culture suspended in either 5 percent hog gastric mucin or broth. A brief discussion of the words standardized culture would seem to be beneficial. In order to obtain reproducible results with a test compound it is necessary to control, as much as possible, the many variables that can enter into this type of test. An organism of high virulence if used in large enough numbers can make almost any drug look inactive. On the other hand, an inoculum not able to procduce a measurable difference between treated and untreated groups is equally lacking in purpose.

Stock cultures of test organisms are normally maintained on slants or in liquid medium. When not routinely used they are maintained at refrigerator temperature or in a lyophilized state. When it becomes necessary to use a culture in animal protection tests the culture is suspended in a volume of saline or broth, and the density of the suspension is measured by a photoelectric colorimeter. From this stock, lO-fold dilutions are prepared. Each dilution is inoculated into a series of mice in order to determine the LD the LD being the lowest concentration of organisms required to produce 100 percent deaths. For example, if it is found that a dilution of l is the lowest level of organism that will produce 100 percent death, we would probably use an inoculum of l0 for the drug evaluation experiments. This means that we are using about 10 LD or 10 times the minimum dose required to kill mice. Such a test would also include the use of control animals which receive an inoculum of 10 l0 and possibly 10. These dilutions are included as'a check on possible variation in virulence which can occur. Having previously determined, through the virulence titration, that 10 was the maximum dilution that will kill we naturally expect these animals to die, usually within 24 hours.

Each organism has its own standardized inoculum level. Some, such as Staphylococcus, may be used at 10", while others like Streptococcus require weekly animal passage in order to maintain virulence.

When evaluating an antibiotic for its effectiveness after a single dose, the dose is usually administered 0.5 hours after inoculating the mice with the lethal concentration of organisms. In this type of treatment schedule surviving mice are usually held for 4 days after the treatment and the percent alive is calculated.

Other methods of administration of the useful products of this invention to animals include mixing with animal feeds, the preparation of feed concentrates and supplements and dilute solutions or suspensions, e.g., a 0.1 percent solution, for drinking purposes. Surprisingly, the addition of low level amounts of the herein described quinoxaline-di-N-oxides to the diet of healthy animals, both ruminant and non-ruminant, such that these animals receive the product over an extended period of time, at a level of from about O.l mg./kg. to about I00 mg./kg. of body weight per day, especially over a major portion of their active growth period, results in an acceleration of the rate of growth and improves feed efficiency (the number of pounds of feed required to produce a pound gain in weight). lncluded in these two classes of animals are poultry (chickens, ducks, turkeys), cattle, sheep, dogs, cats, swine. rats, mice, horses, goats, mules, rabbits, mink, etc. The beneficial effects in growth rate and feed efficiency are over and above what is normally obtained with complete nutritious diets containing all the nutrients, vitamins, minerals, and other factors known to be required for the maximum healthy growth of such animals. The animals thus attain market size sooner and on less feed.

The herein described feed compositions have been found to be particularly valuable and outstanding in the case of swine. In some instances the degree of response may vary with respect to the sex of the animals. The products may, of course, be administered in one component of the feed or they may be blended unifonnly throughout a mixed feed; alternatively as noted above, they may be administered in an equivalent amount via the animal s water ration. It should be noted that a variety of feed components may be of use in the nutritionally balanced feeds. Any animal feed composition may be prepared to comprise the usual nutritional balance of energy, proteins, minerals and vitamins together with one or more of the quinoxaline-di-N-oxides described above. Some of the various components are commonly grains such as ground grain and grain byproducts; animal protein substances, such as meat and fish by-products, vitamin-aceous mixtures, e. g., vitamin A and D mixtures, riboflavin supplements and other vitamin B complexes; and bone meal, limestone and other inorganic compounds to provide minerals.

The relative proportions of the present compounds in feeds and feed concentrates may vary somewhat, depending upon the compound, the feed with which they are employed and the animal consuming the same. These substances are advantageously combined in such relative proportions with edible carriers as to provide pre-mixes or concentrates which may readily be blended with standard nutritionally balanced feeds or which may be used themselves as an adjunct to normal feedings.

In the preparation of concentrates a wide variety of carriers, including the following: soybean oil meal, corn gluten meal, cotton seed oil meal, sunflower seed meal, linseed oil meal, cornmeal, limestone and corncob meal can be employed. The carrier facilitates uniform distribution of the active materials in the finished feed with which the concentrate is blended. The concentrate may be surface coated, if desired, with various proteinaceous materials, or edible waxes, such as zein, gelatin, microcrystalline wax and the like to provide a protective film which seals in the active ingredients. It will be appreciated that the proportions of the drug preparation in such concentrates are capable of wide variation since the amount of active materials in the finished feed may be adjusted by blending the appropriate proportion of concentrate with the feed to obtain the desired degree of supplementation. In the preparation of high potency concentrates, i.e., pre-mixes, suitable for blending by feed manufacturers to produce finished feeds or concentrates of lower potency, the drug content may range from about 0.1 g. to 50 g. per pound of concentrate. The high potency concentrates may be blended by the feed manufacturer with proteinaceous carriers, such as soybean oil meal, to produce concentrated supplements which are suitable for direct feeding to animals. The proportion of the drug in these supplements may vary from about 0.] to 10 g. per pound of supplement. A particularly useful concentrate is provided by blending 2 g. of drug with 1 pound of limestone or 1 pound of limestone-soybean oil meal l l Other dietary supplements, such as vitamins, minerals, etc., may be added to the concentrates in the appropriatecircumstances.

The concentrates described may also be added to animal feeds to produce a nutritionally balanced, finished feed containing from about 5 to about g. of the herein described compounds per ton of finished feed. In the case of ruminants, the finished feed should con tain protein, fat, fiber, carbohydrate, vitamins and minerals, each in an amount sufficient to meet the nutritional requirements of the animal for which the feed is intended. Most of these substances are present in naturally occurring feed material, such as alfalfa hay or meal, cracked corn, whole oats, soybean oil meal, corn silage, ground corn cobs, wheat bran and dried molasses. Bone meal, limestone, iodized salt and trace miner.- als are frequently added to supply the necessary minerals and urea to provide additional nitrogen.

As is well known to those skilled in the art, the types of diets are extremely variable, depending upon the purpose, type of feeding operation, species, etc. Specific diets for various purposes are listed by Morrison in the Appendixof Feeds and Feeding, the Morrison Publishing Company, Clinton, Iowa, 1959.

In the case of non-ruminant animals, such as hogs, a suitable feed may contain from about 50 to 80 percent of grains, 3 to 10 percent animal protein, to 30 percent vegetable protein, 2 to 4 percent of minerals, together with supplementary vitaminaceous sources.

In practice, growth promotion is determined in swine, for instance, by the method wherein young pigs from 5-6 weeks of age and averaging 2L5 lbs. initial body weight are maintained on an ad libitum consumption of water and feed formulation consisting of ground yellow corn-(58.1%), soybean meal (l9.6%), alfalfa meal (2.0%), dried skim milk (5.0%). dried whey l0.0"/2 stabilized animal fat (2.5%), limestone (0.6%), dicalcium phosphate (l.l%), iodized salt (0.5% vitamin premix PPMS (0.5%), quadruple delamix (0.05%), and zinc carbonate (156 g./2,000 lbs. mix). The pigs are divided into groups of 32 pigs each and are held for a pre-experimental period of three days prior to starting the experiment. The quinoxalinedi-N-oxides of the present invention are added to the feed at a ratio of 50 g. of compound per ton of feed. After 28 days the efficacy of said added compounds on growth promotion is measured by a comparison of the growth in terms of weight gain of the treated animals with the untreated control group, which is arbitrarily assigned a weight gain Index of I00. For example, if a compound effects a growth 23 percent greater than the control group (with an Index of 100) it would be assigned a value of 123, etc.

, The effect of a supplemented diet on the growth promotion in other species of animals using appropriate dose levels and feed formulations is assessed in an analogous manner.

The following examples are provided solely for the purpose of illustration and are not to be construed as limitations of this invention, many variations of which are possible without departing from the spirit or scope thereof.

EXAMPLE I acid, 2-

16 anhydrous sodium sulfate and concentrated in vacuo to dryness. The residual solid is triturated with ether, filtered and dried, 179 g., m.p. l3l-133 C., with decomposition.

fOI' C14H14O5N2: 9.2. Found: C, 54.7; H, 4.7; N, 9.1.

C, 54.9; H, 4.6; N,

EXAMPLE ll 3-Methyl-6 and 7-chloro-2-quinoxalinecarboxylic Acid, 2-(acetyloxy)ethyl Ester, l,4-dioxide.

To 25.5 g. (0.15 mole) of S-chlorobenzofuroxan and 28.2 g. (0.15 mole) of 2-acetoxyethyl acetoacetate in ml. of dimethylformamide at 50 C. is added dropwise 30 ml. of ethanol containing 345 mg. of reacted sodium metal. The resulting reaction mixture is allowed to stir at 50 C. for an additional 4 hours followed by the removal of the solvent under reduced pressure. The residual darkoil is dissolved in a minimum amount of chloroform and placed on a column containing 250 ml. of silica in ethyl acetate. The first 300 ml. of the eluate, benzene, is discarded and the subsequent 1.4 liters collected and concentrated to an oil. Trituration with ether results in crystallization of the desired product, which is purified by recrystallization from ethyl etherhexane, 3.74 g.

EXAMPLE Ill The procedures of Examples 1 and II are repeated, using equivalent amounts of the requisite starting benzofuroxans and acetoacetates, to produce the following waist CHa( t)ICO2- CHaCOr HCO (CHahCHCO CH;(CH1)rCOa- Cl Same as above CHaCOr- Cl ..d0 H0O:- CL. dO CHgCHzCOr- 01.. C zC 1- CHJCOT' CL. CH2CH2- HCO: CL. CHaCHz-.-. CH CHQCO Br -(CH:)4 CHaCOQ- Br (CH2)4- HC02- Br. -(CH:)4 CHgCHzCOr -CH-(CHi)a CHJCOT- B1- Same as above H00;- Bl' .(10 (CH;):CHCO Br -CHzC(CHa)zCH2--. HCOz- Br. 1 Ha)2 Hz-. F-.. 2 a)i Hr F... 2 J)2 2- CH;(CHi)zCO2- F -CH(CHa)CHtCH(CH CH (CH2)rCOz- F CH2 0113607- Ci Same as above CH CO Cl CHCH 0 CaH1 X A Z 01 Same as above HCO Br C H(CH;):- CHaCOI- Br Same as above HO,-

EXAMPLE [V The procedures of Examples I and ll are again repeated. using the appropriately substituted benzofuroxan and acetoacetates to provide the following analogs: I

COz-A-Z X N CH:

' X A Z -CHzCHz-.. HCOZ CHaClIzCOz- CH3(CI'I2)2CO2 (CHflzCHCOz- CHaCOz- CHaCHzCOr HCO2- HCOz- CH3CO2 CHsCHzCOr- HCOr- CHaCOz- (CH3)zCHCO2- CH3 CH(CHz)z (CH3)2CHCO2 CH: Same as above CH3CO2 CHaCO2- CHaCO2 Ha Ha Same as above H002- O... HCO

CHgCHg CH3(CH2)2COz- -CH2CH2 CH3CO2 CHzCHz HCO2 -CH(CH2)2- CHaCOz- Same as above.

CHaCOrhydroxyethyl ester, l,4-dioxide To 100 ml. of a l2N hydrochloric acid solution is added 50 g. (0.16 mole) of 3-methyl-2- quinoxalinecarboxylic acid, 2-(acetyloxy)ethyl ester, l,4-dioxide, and the resulting reaction mixture allowed to stir at room temperature'for 1 hour. Two hundred milliliters of water and 200 ml. of chloroform are added to the mixture which is then cooled and adjusted to pH 5, using a ll) percent sodium hydroxide solution. The

mixture is further extracted (4 X ml.) with chloroform, and the combined chloroform layers dried over anhydrous sodium sulfate and concentrated to dryness under reduced pressure. The resulting residue is recrystallized from chloroform hexane to provide the pure product as a yellow solid, 33.3 g., m.p. l46-148 C.

Anal. Calcd. for C l-l O N C. 54.5; H, 4.6; N.

10.6. Found: C, 54.2; H, 4.5; N, 10.7.

EXAMPLE VI The procedure of Example-V is repeated, using the appropriately substituted quinoxaline-di-N-oxides, to provide the following analogs:

EXAMPLE Vll Found: C, 47.8; H, 4.8; N, 13.9

EXAMPLE vm The procedure of Example V" is repeated, using the appropriately substituted starting materials, to provide the following congeners:

CF: -CH(CH;)

5 CF; CH-C-H L Ha (EH;

on ort-omits EXAMPLE lX EXAMPLE X The procedure of Example lX is repeated, employing the requisite starting materials, to provide the following products:

using the appropriate starting materials, to produce the following congeners:

(dimethylamino)ethyl ester, 1,4-dioxide hydrochloride To a solution of 204 g. 1.5 moles) of benzofuroxan and 259 g. (1.5 moles) of 2-(dimethylamino)ethyl acetoacetate in 600 ml. of dry dimethylformamide is added over a minute period 150 ml. of l N sodium ethoxide in ethanol (0.15 mole). The resulting exothermic reaction raises the reaction temperature to 55 C. where it is maintained for 3 hours after the rection subsides. The solvent is removed under reduced pressure, 1 liter of water is added and the product extracted with (5 X 200 ml.) methylene chloride. The combined methylene chloride extracts are extracted with (3 X ml.)

- 0.5 N hydrochloric acid andthe combined aqueous acid layers basified with potassium carbonate and reextracted with (4 X 200 ml.) methylene chloride. The methylene chloride extracts are combined, dried over anhydrous sodium sulfate and concentrated in vacuo to an oil. The residual oil is dissolved in 500 ml. of methanolic hydrogen chloride, and the resulting solution cooled. The desired product crystallizes as the hydrochloride salt, which is filtered, washed with ethyl acetate and dried, 138 g., m.p. 192 C. with decomposition.

Anal. Calcd. for C l-l 0 N Cl:

12.8 Found:

C, 51.3; H, 5.5; N,

C, 51.2; H, 5.7; N, 12.8

EXAMPLE Xlll 3-Methyl-2'quinoxalinecarboxylic acid, 3- (die thylamino)propyl ester. 1,4-dioxide hydrochloride; m.p. 144l45 C.

3-Methyl-2-quinoxalinecarboxylic acid, 1-methyl-2- (diethylamino )propyl ester, 1,4-dioxidc; m.p. 104-l05 C.

EXAMPLE XlV The experiment conditions of Example X11 are repeated, using the requisite starting materials, to pro duce the following compounds: 1

23 24 EXAMPLE XV o 3-Methyl-2-quinoxalinecarboxylic acid, Z-aminoethyl s ester, l.4'dioxide hydrobromide X To a solution of 3.0 g. (0.01 mole) of 3-methyl-2- CH; quinoxalinecarboxylic acid. Z-aminoethyl ester, 1,4- f dioxide hydrochloride dissolved in ml. of water at 0 0 C. is added 1.25 g. (0.005 mole) of silver oxide. The X A z s. aureus E. coli suspension is allowed to stir briefly in the cold followed H 12 5 50 by centrifugation of the solid suspension. The supernal 0 013cm tent solution containing the free base IS treated with 1.7

. Cl (CH2)z- O 3.1 100 g. (0.01 mole) of a 48 percent hydrobromlc acid and 1 the resulting solution concentrated to dryness in vacuo.

H CH N CH H01 100 6.25 The resulting hydrobromlde salt is triturated with ethyl CI 4 3:: gh 12' 5 12 5' acetate and suction filtered. l 5 H (CH2)2- N(C2H5)2-HC1 100 12.5 r 7 01 (CH2)2 N(C2H5)2'HC1 12.5 12.5 H -(CH2)2 N(CH3)C2H5-HC1 100 12.5 g i$riiii on i iig ii c i 388 ibii EXAMPLE X c1 -cH oH oHT N(CH:): HCl as 200 Employing the procedure of Example XV the hydro- II --(CH2)2 o 25 200 chloride salts of the products of Example Vlll are con- ()(|NHC:H5 velalrtedhto their respective hydrobromide, sulfate and H 200 200 p ate 5a o-dwamooat EXAMPLE XVII a w v I Y Employing the aforementioned two-fold serial dilu- EXAMPLE tion technique, the in vitro activity of some of the prod- Using the previously described method for determinucts of the instant invention against Staphylococcus auing in vivo activity, the following compounds were reus and Esclzerechia coIi are presented. Benzylpenitested orally against Strepmc'uccus pyogcnes at 200 and cillin (K Salt) when tested gave MIC (Minimal lnhibimg./kg. and against Esc/iercchiu coli at 100 and 25 tory Concentration) values of 0.156 and 100 vs. S. mg./kg.. unless otherwise indicated, the results being aureus and E. coli, respectively. recorded as the percent animals which survived:

-co2A-z o CHa S. p uge'nex In. coli x A z 200 50 100 25 H a, I i z)2- I) so so 100 so -()-(E()llz o1 cm 2- 100 so m -o-ccrh H (CH2)2 -OH 100 100 30 H -(CH2)2- NH1-HC1 90 a0 100 H --(CH2)z N(CH3)2-HC1 20 100 40 H -(CH2) N(CH3)C2II5-I Cl 100 so so 0 1r (CH2)a- N(CH3)Q-IIC1 mo 20 so 40 H -CH(CHa)CH2 Nicaragua so 20 00 0 H (CH;): (6 100 100 OCC2C1 H (CH2)2- no 1 40 ll O-CCzN(CHa)aCl- H -(CH2)2 H 30 2 40 OCNHC2H5 H (CH:)2 1 100 1 7o OCCIHI H -(CH:)2 H 3o II (CH1)2- -OCOCII;CII(CII3)2 1r -(oHmc 1 so -O-CCH2CHJ n (CH;)2 0 m 30 bonate (7.8 g./ 100 lbs. mix). All the pigs, which are divided into groups of six, are inoculated on day with 4 ml. (approximately 2.0 X 10 organisms) of the stock inoculating suspension. Treatment with the quinoxaline-di-N-oxides of the present invention is carried out on day 0 and day l by intramuscular injection at 12 hour intervals at doses of 2.5 and mg./kg. On day the percent mortality in each group is calculated. The following results are obtained:

Employing the previously described procedure for determining growth promotion in animals, the following quinoxaline-di-N-oxides were tested at 50 g./ton of feed in swine for a period of 28 days and provided the following results:

S. pyogenes E. coll X A z 200 50 100 25 II (C1I2)r 1 100 so -oi :(cH2),oH3

H -(CH2)2- 1 9O 7 0 -0--ii1(CH2)4CH3 H --(CHz)z- 0 1 100 I 10 -O--( il(CHz)sCH3 H -(cHm- 1 100 1 so O--( (CHz)eCHa H -(CH2)- 1 100 I -o-i 3(oHz)8oH3 H (CH:)'. 1 100 I so -oii(cHQ)zco1H 11 (CH:):- o 50 -0-ii(oH,)1oo2H 1 200 mgJkg. 2 5O mgJkg.

7 EXAMPLEXiX M A Egg;

The efficacyof the herein described compounds in 25 X A Z protecting against a systemic challenge infection of Salmonella cholerasuis var. kunzendorf in swine is dem- H "(0111)? 129 29 onstrated by the following experiment. Young, 6-8 H (CH2): 0 153 53 week old pigs are conditioned for 14 days in isolation -assist.ssazszzzifgssszs332.21%a:1 39 soybean meal (15%), alfalfa meal (2%), meat bone scraps (2.5%), limestone (0.4%), dicalcium phosphate (0.65%), iodized Salt (0.5%), Vitamin pre-mix PPM M V A w No. 5 (0.5%), quadruple delarnix (0.05%) and zinc car- 35 EXAMPLE XXI The experimental procedure of Example I is re peated, using the appropriate B-ketoesters and benzofuroxans. to provide the following compounds:

EXAMPLE xxu Employing the procedure of Example V and starting with the requisite quinoxaline-di-N-oxide, the following analogs P pa ed; v a

EXAMPLE XXlll Starting W EEBRQRQQ QqEE QQl idi-btqxt s and following the procedure of Example VII, the folw sqn sws are P ep EXAMPLE XXIV The procedure of Example lX is again repeated,

using the requisite starting material, to provide the. following analogs; 7 v

EXAMPLE XXV EXAMPLE XXVI 3-Methyl-Z-quinnxalinecurhnxylic acid, (acetoxy)ethyl ester, l,4-dioxide via Scheme l To a solution of 13.6 g. (0.1 mole) of benzofuroxan and 18.8 g. (0.1 mole) of 2-acetoxyethyl a-ketobutylrate in 150 ml. of ethanol is added 1.36 g. (0.02 mole) of sodium ethoxide. The resulting reaction mixture is stirred at 50 C. for hours, and is then filtered while warm, and concentrated under reduced pressure. Cooling and scratching results in the crystallization of the desired product, which is identical to that isolated in Example 1.

EXAMPLE XXVll 3-Methyl-2-quinoxalinecarboxylic acid, 2- (dimethylamino)ethylester, 1,4-dioxide via Scheme 11 To 250 ml. of toluene is added 27.2 g. (0.2 mole) of 3-methyl-2-quinoxalinecarboxylic acid, methyl ester, 1,4-dioxide, 17.8 g. (0.2 mole) of dimethylaminoethanol and 37.8 g. (0.22 mole) of p-toluenesulfonic acid and the resulting mixture is heated to reflux. During the reaction period the reflux condenser is removed and the toluene is allowed to boil off, removing methanol, the by-product of the reaction. When the reaction is complete the solvent is removed in vacuo and the residue partitioned between water and chloroform. The water layer is then separated and made slightly basic by the careful addition of a sodium hydroxide solution. The basic solution is then extracted with methylene chloride, dried over sodium sulfate and the solvent removed under reduced pressure. Addition of methanolic hydrogen chloride to the residue provides the hydrochloride of the desired product, identical to that prepared in Example X11.

EXAMPLE XXIX The procedures of Examples XXVI, XXVll and XXVlll representing Schemes 1, 11 and 111, respectively, are repeated, using the appropriate starting reagents and reaction conditions, to provide the following quinoxaline-di-N-oxides:

i N C0 -A-Z N/ RI EXAMPLE xxx 3-Methyl-2-quinoxalinecarboxylic acid. 2-(4-carboxypropionyloxy)ethyl ester, 1,4-dioxide A mixture of 26.4 g. (0.1 mole) of 3-methyl-2- quinoxalinecarboxylic acid, 2-hydroxyethyl ester. 1,4- dioxide, 22 g. (0.22 mole) of succinic anhydride and ml. of triethyl amine in l l. of acetone is heated to reflux for 2 hours and then allowed to stir at room temperature for 16 hours. The resulting reaction mixture is poured into water and extracted with chloroform (3 X 250 ml.). The aqueous phase is acidified with concentrated hydrochloric acid and the product extracted with chloroform. The organic layer is separated, dried over sodium sulfate and concentrated to an oil under reduced pressure. The residual product is induced to crystallize from methanol, 24.7 g., m.p. -167 C.

Anal. Calcd. for C H O N z C, 52.8; H, 4.4; N,

Found: C, 52.9; H, 4.6; N, 7.6.

By substitution of glutaric anhydride for succinic anhydride in the above example 3-methy1-2- quinoxalinecarboxylic acid, 2-(4-carboxybutyryloxy)- ethyl ester, 1,4-dioxide, m.p. ll41 17 C., is isolated.

Anal. Calcd. fOI C]7H1808N2: C, H,

Found: C, 53.7; H, 4.8; N, 7.4.

EXAMPLE XXXl (octanoyloxy )ethyl ester, l',4-dioxide W U EXAMPLE XXXll 3-Methyl-2-quinoxalinecarboxylic acid, 2-

31 32 To a solution of 26.4 g. (0.1 mole) of 3-methyl-2- x A z qumoxalmecarboxylrc acid, 2-hydroxyethyl ester, l,4- We dioxide and 30.3 g. (0.3 mole) of triethyl amine in 200 Br -(CH, ccH,B'r ml. of methylene chloride and cooled to 0 C., is added OQCHflrCHfl dropwise 32.5 g. (0.2 mole) of octanoyl chloride in 50 E; :3 :ggg:g:i. cri.rci+. ml. of the same solvent. After stirring at 0 C. for 1 hour Br -cH(C, .n( t)= the reaction mixture is allowed to warm to room temg: :gfggfg perature and is subsequently washed with a saturated c n QH(CH C:l l, -'o,ccH c r 7 sodium bicarbonate solution and then with 6N hydro- 5: -g:g: ;g:= g g g= gg chloric acid. The organic layer is separated, dried over 10 z igf magnesium sulfate and evaporated in vacuo to an CHa -CH. .CH,- O=COC. .H amber oil which is induced to crystallize with ether -0,CCH(CH ,)cH,Br troleum ether 25 7 m 62-64 c CHMF P r -P- ocH (cH,),- -o,ccHc|cH,

By replacing octanoyl chloride with the appropriate $33 g:)= 8g =)2 H acid chloride, the following compounds are similarly l5 Z & prepared: oCH CH CH(C H )CH 0 Co cH ,CH, 3-methyl-2-quinoxalinecarboxylic acid, 2- 85:: 8:g :fgfi

I a (prop1onyloxy)ethyl ester, l,4-dioxide, m.p. 95 C.', 3- ocl-r -cH,cH(c,H,)cH,- o,ccH(c,H,

C -(CH -o COCH CH(CH methyl 2 quinoxalmecarboxyh c I acid, 0 O 2 1 0:C(CH:)CHn 1 1 (butyryloxy)ethyl ester, l,4-dioxide, mp. 68 69 C.; Q2CCHB,CH2CH1 3-methyl-2-quinoxalinecarboxylic acid, 2-

g::h 8:g zh .-r (valeryloxy)ethyl ester, l,4-dioxide, m.p. 56-60 c.; 1 ,31: I S T 3-methyl-2-quinoxalinecarboxylic acid, 2- CFn -(CH,),- -0,CCH(c'H.1)(CH),CH, (caproyloxy)ethyl ester, l,4-dioxide, m.p. 7072 C.; *(CHHP 3-methyl-2-quinoxalinecarboxylic acid, 2- (enanthyloxy)ethyl ester, l,4-dioxide, m.p. 6870 C.; 3-methyl-2-quinoxalinecarboxylic acid, 2- EXAMPLE XXXlV O O (capryloxwethyl i f 62 3-Methyl-2-quinoxalinecarboxylic acid, Z-(N-ethylcar- 3-methyl-2-qumoxalinecarboxyl|c acid, 2- b amyloxy)ethyl ester, l,4-dioxide (chloroacetoxy)ethyl ester, l,4-dl0Xide, m.p. 0

o o A solution containing 5.28 g. (0.02 mole) of 3- H5 J17 3-mcthyl'2qumoxalmeearbaxime acid methyl-2- uinoxalinecarbox lic acid "-h 'drox eth l 2-[(ethoxycarbonyl)oxylethyl ester, l,4-dioxide; and y y .y y

ester, l,4-dioxide and 5 ml. of ethyl isocyanate m 100 3-methyl-2-qumoxalmecarboxylrc acid, 2- 0 ml. of dry methylene chloride is heated to 40 C. for 8 I(lsobutoxycarbonyl)oxyl-ethyl ester, l,4-dioxide, h Th l m p C ours. e resulting reaction mixture is partitioned between water and ethyl acetate, and the organic phase EXAMPLE XXXU] separated, dried and concentrated to a yellow solid. The mcedure of Exam le XXX is re eated The desired product is purified by recrystallization p p p from chloroform ether, 5.26 m. ll6l 18 C.

8 P ploymg the requisite acid halides and hydroxyalkyl esters of 3 methyl 2 qumoxalmecarboxylic ac d, l,4 Anal. Calcd for CMHHOGN: C, 53.7; H 51; N, dioxide of Example Vl, to provide the following ana- 12 5 logs: Found: c, 53.5; H, 5.1; N. 12.3.

0 EXAMPLE xxxv co A-Z x Starting with the appropriate 3-methyl-2- quinoxalinecarboxylic acid, hydroxyalkyl ester, 1,4- dioxide and alkyl isocyanate, and repeating the proce- A 97 V dure of Example XXXlV, the following quinoxalinel,4-dioxides are 5 nthesized: A Z H X o l x y A z x A 2 Br CH(CH=)(CH;) -0,CNHCH3 F (CH,)= oicmcnnm cgmi Br -'-CH(CH,)(CH,)3 O- .CNHC H F --(CH=l,- 0,CCH((H )N(i-C ,H Br CH(C,H5)(CH): O,CNH-iC,H, F (CH,)= O-,,C((H=),N(CH=,)2 CH3 (CH=),- O=CNHC2H5 5 F (CH=),- O=C(CH N(CH;,)C H CH3 CH(CH;,)CH; O CNHnC H F *CH:C(CH.1):CH: 2 a 7) CH -CH(CH,)CH,- -o,CNH-t-C.H, F CH,C(CHJ)2CH,- -0,CCH, N(CH,)= CH; CH(CH3)(CH2)2- .O,CNHCH3 F CH=C(C .1).C 2 2 :i 7): CH3 CH(CHJ)(CH2)2 O1CNHCH5 Cl -CH(C2H.\)CH2 '-O:CCH(CH:A)CH:N(CH:J)2 OCH; CH2C(CH3)2CH2 0=CNHC,H5 Cl n) 2- a s)z ocu, -cH,c(cH,),cH, O,CNH-sC l-l, =)1 2 s) 2 n)2 OCH; CH2CH(C:H5)CH2 O2CNHCZH5 10 g )2 ggg((2g;;g:gu- :a 1 OCH CH -o CNHCH r -i ocni JeniocNn i c,H, Br (CH: -0:CCH:N(C,PL): 5 CF; (CH2) 02CNHCH3 '(CH2)4 z :)a 3)2 CF; (CH)2 O,CNH-SC4 H9 z s)( 2)z z( z): s)2 cF CH CH(CH )(CH -0 CNHs-C H CH 3 CH(CH=)CH2 -O CCH,N(iC H,),

ai r -8=fifi*li3*5i 3 (CH2)2 -O2CCH(CH )CH N(CH )2 EXAMPLEXXX 22: we aestetianfieie 3-Methyl-2-qumoxalinecarboxyltc acid, 2-

(CC! lH2:)El CH CH 8ZEESE(C:2IL;JN((CH)ZZ 5 H.) H (dimethylam noactetoizy)eth(;l0ester, 11,4 dtlO3X1d h l2 CF 2 2 c ii N ii & i-i,) To a solution 0 g. 1 mo e) 0 -met y- CF3 OZCCH(,, CBH7)N(CZH5)Z qumoxahnecarboxyhc acid, 2-(chloroacetoxy)ethyl es- 15:2)? -geg(gge)iz(g fi)n ter, l,4-dioxide in 40 ml. of methylene chloride is Q 31: I Z E SZ E LEZQ added, with cooling, l.0 g. (0.022 mole) of dtmethyl amine in ID ml. of the same solvent. The reaction mix- M e e ture is allowed to stir for several hours at room temper- 25 EXAMPLE XXXVI ature and is then heated to 40-50 C. for an additional 3 z i i i acid, 2- hOUl'. The mixture l-S poured Into water and the organlc I(dimethylamino)acetoxylethy] ester l 4-dioxide phase separated, dried over sodium sulfate and concen methyl hl i trated to dryness. The crude product is purified by re- Method A cryslauizalio" f Chlomform T To a solution of 681 mg. (2 m moles) of 3methyl-2- A small P of the free base quinoxalinecarboxylic acid, 2-(chloroacetoxy)ethyl esform and sufficlem y q chlorlde ter, 1,4-dioxide in 25 ml.ofmethylene chloride is introethyl acetate added to precipitate the hydrochloride d d i h l amine gas i h 1i (0 10C f salt. 5 minutes. The reaction mixture, which is ke t at 0 C.

P EXAMPLE XXXVI! overnight, IS filtered and the solids washed with aceo Employing the procedure of Example XXXVI, and tone and dried, 570 mg. mp. 85 C. The product is starting with the appropriate secondary, dialkylamine used furthfir Punficatlonand bromo or chloro compound from Example M f 3 5 0 0 l f 3 h I XXXlll, the following tertiary amines are prepared: a f 0 1 m0 0 3 40 qumoxalmecarboxylic acid. 2-

0 (dimeth laminoacetox )eth l ester, 1,4-dioxide in 40 1 y y y N COFPZ ml. of cooled (O5 C.) methylene chloride lS slowly X added over a period of 15 minutes methyl chloride gas. The reaction is ke t at 0 C. for several hours and is CH: v p

f then allowed to warm to room temperature. The solid Q A suspension is filtered, washed with acetone and oven X A 2 dried. The product is identical to that prepared by the above-described Method A. H -(CH2)- O:CCH- .N(C,H,,)2 H (CH:): g H(C u) :|)2 H -(CH2) 1 O2CCH,N(CH,)C2H "(CH:): 2 .1 Starting with the a propriate reagents and followin H (CH) 0-CCH N(CH g H i the procedure of either Method A or B of Example #CHiCH-OCHF... eqectcuaeueceaae W 9 the (flo ng quater ry a a pr p COn-AZ CH N 3 Method x A Z Anion Salt A H (CH- 0,CCH(CH )N*(CH Br B H (CH,), -o,CCH,N*(CHn)( e e)n B H -(cH,) O,CCH2N"(CH3)2(CH5) CHJSO3 A H -(CH,) 0,CCH N(C= n)n B F CH,C(CH,),CH,- O CCH,N*(CH )=(nC H,) Cl B F -CH,C(CH,),CH, O,CCH,N (CH=')(n--C H 2 E (cH,),- (CH,)n *(CHn)n B C Method X A Z M W Anion Salt A Cl -(CHZ): O,CCH(C,H,.)CH2N(CH3)3 Cl A 3; CH, --0,CCH,N*(i--Cn 1la B Br -(CH)4 ozc(C :|)z( 2 5) A Br CH(C,H,,J(CH,), O,C(CH:)z :|)n B CH CH(CHH)CH, O,CCH,N*(CH )(iC H,), l- 8 CH3 -(CH,), -0, CCH(C,H,)N*(C,H5),(CH 1) CH SQ; A CH, -(CH,),- O,CCH(C,H,)N*(C,H,) Br A CH, cH(cH cH,- -O,CCH,N*(n-C H,), Cl A OCH, -(CH,), O,CCH(CH3)N(C,H,,),(CH,,) Cl A OCH, (CH,) O,CCH(C,H,)N(CH;,)3 Br 5 OCH; (CH,),, O,CCH(C,H,,)N"(CH:) 1 CGHSSOJ A OCH, CH,CH(C,H,)CH,- O,CCH(CH )N*(CH 1)=(n-C;,H,)

cl- B CF" -((H,),-- -O,CCH(nC H,)N*(C,H,),(CH=) 8 CF: '(CH:): 2 1) s)z( :i)

CHJSOJ B CF: (CH:): s) 1)z( a) A ck (Cl-l,).- O,C CH,).N (CH,), Br A CF (CH=) -O,C(CH,).N (CH,),(C,H,) Br B CF, -(CH,).- -0,C(CH,)4N*(CH3) ,(nC,H-,) cr

W EXNWPL XL m I A Z 3- z I i pji div 7 V i Methyl 2 qumox ilmecarboxyhc d Cld 2 (3 CH(CHH)CH2 CHnCHzCOP carboxypropionyl-oxy)ethyl ester. l,4-d1ox1de Sodium Salt CH:CH(CH) ("inco A suspension of 728 mg. of 3-methyl-2- 15:31:: fififi g quinoxalinecurboxylic acid, 2-(3-carboxypropionylox- (CH CH -.CH2CO, -(CH2) (CH, cutoy) ethyl ester. 1,4 dioxide in 50ml. of water IS treated CH(C;{H)(CHM with one drop of phenolphthalem and then neutralized CH(CH;|HCH,]2 icH..)2CHco with lN sodium hydroxide solution. The hazy solution E SE gfi gg is subsequently filtered and evaporated to dryness cH,c Hic,H:;- 6 1 under reduced pressure. 665 mg., mp. 148 C. (.Ht)s C .-.CO,

In a similar manner, the corresponding acids of Example XXX] are converted to their pharmaceutically CH(CH:1)(CH=l 1 (CH.. ,cHco, acceptable basic salts. EE EFSj-Qffi' figb 2 il 2 2' 1 CH,C(CH CH,- CH co Preparation A g: g :gn, CHZCHIZCOZ Acetoacetic Acid, Alkanoyloxyalkylene Esters f ii fi r f nt ta a. 2-(Acetyloxy)ethyl acetoacetate CH(CH,1)CH2CH(CH3)- To a stirred solution of 500 g. of Z-hydroxyethyl ace- CHHCOF tate and 1 ml. of 12 M sulfuric acid is added 403 g. of 40 :t) 2 n) diketene at such a rate that the reaction mixture temxggfg perature is maintained at 85l05 C. When the addi- CHJCOQ-I tion is complete the mixture is heated to 1 10 C. for 30 :gfigfi figg p gagl giinutes, and is 3then fractiorgajlly c7lig tiged tgnder re- (c mg::: cri: c

uc re 48 ..l l 1 mm. (CHJCHCO- p f g p -CH cil c HoEii, ncd 2 Genera] YOC CdUI'C. I H2CH(C2H5)CH2 CH3CO2 To the appropriate alkanoyloxyalkanol containing a -g:t% H )C Eigg z)z z catalytic amount of 12 M sulfuric acid is added, apg ij is proximately, an equimolar amount of diketene at such (CH)2 4CH;,C6H4CO, a rate that the temperature of the resulting exothermic 50 Z S $Q CO1 1i 4 reaction remains at about 80-l00 C. The reaction 15 brought to completion by limited heating, 3060 minutes, at l 10-l 15 C. The product is isolated by frac- P tional distillation of the reaction mixture under rereparauon B duced pressure. To a solution of 8.4 g. (0.1 mole) of diketene in 150 The following compounds of the formula CH COCH- ml. of chloroform is added 2i g. (0.11 mole) of 2- CO -AZ are thus prepared: bromoethylamine hydrobromide in 30 ml. of water, and the resulting mixture stirred vigorously at 0 C. A 2 while 4.1 g. (0.1 1 mole) of sodium hydroxide in 20 ml.

of water is added dropwise over a period of 20 minutes CH CH HCO 04:60? The reaction mixture IS allowed to stir at room temper- -gfi=gfi- 23.12 59 ature for 1 hour, after which the chloroform layer is Q I separated, dried over anhydrous sodium sulfate and -:g:1;.1 215%; concentrated to dryness in vacuo to yield the intermej,: cHzcHzcor diate, N-( 2-bromoethyl)acetoacetamide, as a white qgnm- CH=(CH2)=C0, crystalline residue. ilE The above acetoacetamide is added to ml. of eth- CH(CH;,)CH- cHaCo anol containing 13.6 g. (0.1 mole) of benzofuroxan followed by the addition of 16 ml. of ethylamine. The resulting mixture is allowed to stand overnight at room temperature, and is then filtered. The desired product, 2-( 2-oxazolin-2-yl )-3-methylquinoxaline-l ,4-dioxide,

6.4 g., mp. 21 1 C., is further purified by recrystalli- 5 zation from methanol chloroform, m.p. 2172l8 Anal. Calcd. for c.,H,,o N,,;

Found:

C, 58.8; H, 4.5; N,

C, 58.2; H, 4.5; N, 17.0.

Starting with the appropriate bromoalkyleneamine, diketene and requisite benzofuroxan the following compounds of the formula:

Preparation C Acetoacetic Acid, Disubstitutedaminoalkylene Esters a. Acetoacetic acid, dimethylaminoethyl ester To a solution of 230 g. (2.6 moles) of dimethylaminoethanol and 0.5 ml. of 12 M sulfuric acid is added slowly and with external cooling 255 g. (3.0 moles) of ketene at such a rate that the reaction temperature is maintained at 55-65 C. After the addition is complete, which requires l hour, the reaction mixture is allowed to cool to room temperature. Distillation of the mixture provides 371 g. of the desired product, b.p. ll8-l20 C. (l7 mm).

b. General Procedure;

The procedure comprises the controlled addition of diketene to the appropriate disubstitutedaminoalkanol containing a catalytic amount of sulfuric acid. Cooling and addition are adjusted so that the reaction temperature is maintained at about 50-75 C. The product is conveniently isolated by distillation under reduced pressure.

The following compounds having the formula CH COCH CO ANR R are thus prepared:

CH3 CH3 

2. A compound of claim 1 wherein X is hydrogen and A is alkylene having two carbon atoms.
 3. 3-Methyl-2-quinoxalinecarboxylic acid, 2-hydroxyethyl ester, 1,4-dioxide, a compound according to claim 2 wherein A is ethylene.
 4. A compoud of claim 1 wherein X is hydrogen and A is alkylene having three carbon atoms.
 5. 3-Methyl-2-quinoxalinecarboxylic acid, 3-hydroxypropyl ester, 1,4-dioxide, a compound according to claim 4 wherein A is propylene.
 6. 3-Methyl-2-quinoxalinecarboxylic acid, 1-methyl-2-hydroxyethyl ester, 1,4-dioxide, a compound according to claim 4 wherein A is methylethylene.
 7. A compound of claim 1 wherein X is chloro and A is alkylene having three carbon atoms.
 8. A compound of claim 1 wherein X is chloro and A is alkylene having two carbon atoms. 